Police say they received a 911 call about a fire on the fourth floor at 216 West 62nd Street at about 11:35 p.m. on Wednesday.
Sixty firefighters responded to the scene four minutes later and put out the flames, according to fire officials.
Firefighters then discovered the bodies of a 44-year-old man and a 70-year-old woman. Both were pronounced dead at the scene. A third person suffered non-life-threatening injuries.
The fire inside the six-story building is not believed to be suspicious.
The identities of the deceased have not been released pending family notification.