The victim, a 38-year-old woman, was operating the BX1 MTA bus in front of a home along Grand Concourse on May 29, according to authorities.
The suspect, an unidentified woman inside the bus, approached the driver and, apparently unprovoked, pepper-sprayed her in the eyes before getting off the bus and running away.
The victim sustained burning to her eyes and was taken to Lincoln Hospital in stable condition. No-one else was reportedly injured.
Police have released a surveillance photo of the suspect, who is described as a woman in her 30s, standing 5'4" tall and weighing 140 lbs, with a thin build and a dark complexion. She was last seen wearing all black clothing and a black hat.
Anyone with information in regard to this incident is asked to call the NYPD's Crime Stoppers Hotline at 1-800-577-TIPS (8477) or for Spanish, 1-888-57-PISTA (74782). The public can also submit their tips by logging onto the CrimeStoppers website at https://crimestoppers.nypdonline.org/ or on Twitter @NYPDTips.
Police say all calls are strictly confidential.