The attack occurred at 2 a.m. Monday between Madison and Fifth Avenues.
It was unclear what lead to the stabbing. Police were investigating the possibility the stabbing was prompted by a robbery or some kind of dispute.
The man, 27, was struck in the stomach and the hand. He was taken Bellevue Hospital in stable condition.
Two men dressed in all black fled the scene on foot.
In another shocking incident in Midtown, a 32-year-old man, was riding a northbound 2 train entering Penn Station at around 12:17 a.m. when he was stabbed in the neck by an unknown assailant in an unprovoked attack.
The suspect then took off through the station and remained at large. The victim was taken to Bellevue Hospital where he was pronounced dead.